Casp1^em1Stewi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Casp1^em1Stewi
• Name: caspase 1; endonuclease-mediated mutation 1, Stefan Winkler
• MGI ID: MGI:8433190
• Synonyms: Casp1^C284A
• Gene: Casp1 Location: Chr9:5298517-5307281 bp, + strand Genetic Position: Chr9, 2.46 cM
• Alliance: Casp1^em1Stewi page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details:

  Cysteine codon 284 (TGC) was changed to alanine (GCC) (p.C284A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation renders the encoded protein enzymatically inactive. (J:288227)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp1 Mutation: 43 strains or lines available

References

• Original: J:288227 Reinke S, et al., Non-canonical Caspase-1 Signaling Drives RIP2-Dependent and TNF-alpha-Mediated Inflammation In Vivo. Cell Rep. 2020 Feb 25;30(8):2501-2511.e5
• All: 1 reference(s)