Atf4^tm1.2Cmad
Targeted Allele Detail

Summary

• Symbol: Atf4^tm1.2Cmad
• Name: activating transcription factor 4; targeted mutation 1.2, Christopher Adams
• MGI ID: MGI:8401234
• Synonyms: Atf4^gKO(e2/3/pA)
• Gene: Atf4 Location: Chr15:80139385-80141742 bp, + strand Genetic Position: Chr15, 37.85 cM
• Alliance: Atf4^tm1.2Cmad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:379595
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Intragenic deletion
• Atf4^tm1.2Cmad involves 2 genes/genome features (Atf4, Cacna1i) View all
• Mutation details:

  A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette and left exons 2 and 3 floxed. Cre-mediated recombination removed exons 2 and 3. This allele produces a readthrough transcript fusing exon 1 of Atf4 to exon 2 of Cacna1i. (J:206503)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atf4 Mutation: 24 strains or lines available

References

• Original: J:379595 Zhang Z, et al., Transcriptional readthrough at Atf4 locus suppresses Rps19bp1 and impairs heart development. Cardiovasc Res. 2026 Feb 14;121(18):2909-2921
• All: 2 reference(s)