Thrb^em1Lmoe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Thrb^em1Lmoe
• Name: thyroid hormone receptor beta; endonuclease-mediated mutation 1, Lars C Moeller
• MGI ID: MGI:8394035
• Synonyms: Trbeta^GS
• Gene: Thrb Location: Chr14:4431611-4809435 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Thrb^em1Lmoe page

Mutation origin

• Strain of Origin: C57BL/6JCrl

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details:

  Glutamine codon 125 (GAA) was changed to glycine (GGA) and glycine codon 126 (GGC) to serine (AGC) (p.125E_126GdelinsGS) using ZFNs and a donor plasmid template. The mutations, in the first zinc finger of the DNA-binding domain of the encoded protein, abolish the canonical binding of thyroid hormone to this receptor without affecting non-canonical interactions. (J:253749)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thrb Mutation: 42 strains or lines available

References

• Original: J:253749 Hones GS, et al., Noncanonical thyroid hormone signaling mediates cardiometabolic effects in vivo. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11323-E11332
• All: 2 reference(s)