Thra^em1Lmoe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Thra^em1Lmoe
• Name: thyroid hormone receptor alpha; endonuclease-mediated mutation 1, Lars C Moeller
• MGI ID: MGI:8394033
• Synonyms: Tralpha^GS
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^em1Lmoe page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details:

  Glutamine codon 71 (GAG) was changed to glycine (GGG) and glycine codon 72 (GGC) to serine (AGC) (p.71E_72GdelinsGS) using ZFNs and a donor plasmid template. The mutations, in the first zinc finger of the DNA-binding domain of the encoded protein, abolish the canonical binding of thyroid hormone to this receptor without affecting non-canonical interactions. (J:253749)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 40 strains or lines available

References

• Original: J:253749 Hones GS, et al., Noncanonical thyroid hormone signaling mediates cardiometabolic effects in vivo. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11323-E11332
• All: 2 reference(s)