Pfkfb2^tm1Ntang
Targeted Allele Detail

Summary

• Symbol: Pfkfb2^tm1Ntang
• Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; targeted mutation 1, Nan Tang
• MGI ID: MGI:8328376
• Synonyms: Pfkfb2^S468A flox
• Gene: Pfkfb2 Location: Chr1:130616919-130656990 bp, - strand Genetic Position: Chr1, 56.89 cM, cytoband E4
• Alliance: Pfkfb2^tm1Ntang page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:353628
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site, the coding region of exon 15, followed by poly(A) signal sequences, and a second loxP site were inserted into intron 14. The endogenous exon 15 was modified by changing serine codons 468 (AGC) and 485 (AGT) to alanine (GCC) (ENSMUSP00000127587:p.S468A, p.S485A). In the encoded protein, the mutation changes phosphorylatable residues into ones that cannot be phosphorylated. This allele expresses the wildtype protein and only after Cre-mediated deletion of the inserted sequence will it express the mutated protein. (J:353628)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfkfb2 Mutation: 37 strains or lines available

References

• Original: J:353628 Wang Z, et al., Enhanced glycolysis-mediated energy production in alveolar stem cells is required for alveolar regeneration. Cell Stem Cell. 2023 Aug 3;30(8):1028-1042.e7
• All: 1 reference(s)