Redic1^em2Qsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Redic1^em2Qsh
• Name: regulator of DNA class I crossover intermediates 1; endonuclease-mediated mutation 2, Qinghua Shi
• MGI ID: MGI:8323060
• Synonyms: Redic1 KI
• Gene: Redic1 Location: Chr15:91083697-91145097 bp, + strand Genetic Position: Chr15, 46.04 cM
• Alliance: Redic1^em2Qsh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted 2 base pairs into the coding sequence (c.233_234insTT) in exon 4. This mutation results in a frameshift and premature termination (p.M78Ifs*2). It is based on the same human mutation found in azoospermic patients with meiotic arrest. RT-qPCR analysis of Redic1 expression at the cDNA level revealed no degradation of mRNA in testis tissues. Western blot of testicular lysates from 20 dpp homozygous mutant males showed absence of potentially in-frame reinitiation-translated proteins. Immunofluorescence staining showed no REDIC1 signals on chromosome axes of mutant spermatocytes. (J:383089)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Redic1 Mutation: 30 strains or lines available

References

• Original: J:383089 Fan S, et al., A novel recombination protein C12ORF40/REDIC1 is required for meiotic crossover formation. Cell Discov. 2023 Aug 23;9(1):88
• All: 1 reference(s)