Stat5b^em1Wjl
Endonuclease-mediated Allele Detail

Summary

• Symbol: Stat5b^em1Wjl
• Name: signal transducer and activator of transcription 5B; endonuclease-mediated mutation 1, Warren J Leonard
• MGI ID: MGI:8323012
• Synonyms: STAT5B^Y699F
• Gene: Stat5b Location: Chr11:100671557-100741407 bp, - strand Genetic Position: Chr11, 63.63 cM
• Alliance: Stat5b^em1Wjl page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Tyrosine codon 699 (TAC) was changed to phenylalanine (TTC) (p.Y699F) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. In the encoded protein the mutation changes a phosphorylatable residue to one that cannot be phosphorylated, which affects T cell proliferation. (J:353834)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stat5b Mutation: 33 strains or lines available

References

• Original: J:353834 Lin JX, et al., Tyrosine phosphorylation of both STAT5A and STAT5B is necessary for maximal IL-2 signaling and T cell proliferation. Nat Commun. 2024 Aug 27;15(1):7372
• All: 1 reference(s)