Nfe2l2^tm1.1Gne
Targeted Allele Detail

Summary

• Symbol: Nfe2l2^tm1.1Gne
• Name: nuclear factor, erythroid derived 2, like 2; targeted mutation 1.1, Genentech
• MGI ID: MGI:8322213
• Synonyms: Nfe2l2^iE79Q
• Gene: Nfe2l2 Location: Chr2:75505857-75534985 bp, - strand Genetic Position: Chr2, 44.75 cM
• Alliance: Nfe2l2^tm1.1Gne page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:353988
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: The following were inserted into intron 1, 130 bp upstream of exon 2: a loxP site, a copy of the last 130 bp of intron 1, wild-type cDNA coding for exons 2-5, human GH gene 3' UTR, 4x poly(A) signal sequences, an FRT site flanked neomycin resistance gene cassette, and a second loxP site. The neo cassette was removed through subsequent Flp-mediated recombination. Endogenous exon 2 was modified by changing glutamic acid codon 79 (GAA) to glutamate (CAA) (p.E79Q). The mutation is the equivalent of the same mutation found in human lung cancer. This allele expresses the wild-type protein and only after Cre-mediated deletion of the inserted cDNA will it express the modified protein. (J:353988)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfe2l2 Mutation: 94 strains or lines available

References

• Original: J:353988 He F, et al., NRF2 activates growth factor genes and downstream AKT signaling to induce mouse and human hepatomegaly. J Hepatol. 2020 Jun;72(6):1182-1195
• All: 1 reference(s)