Bud31^em1.1Gpt
Endonuclease-mediated Allele Detail

Summary

• Symbol: Bud31^em1.1Gpt
• Name: BUD31 homolog; endonuclease-mediated mutation 1.1, GemPharmatech
• MGI ID: MGI:8288380
• Gene: Bud31 Location: Chr5:145077186-145084888 bp, + strand Genetic Position: Chr5, 85.05 cM
• Alliance: Bud31^em1.1Gpt page

Mutation origin

• Strain of Origin: C57BL/6JGpt

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR-targeting produced an exon 4-5 floxed allele. Exons 4-5 were deleted via cre-mediated recombination in the germline. Semi-quantitative PCR analysis confirmed the absence of transcript in heart, liver, spleen, lung, kidney and testis. (J:333861)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bud31 Mutation: 18 strains or lines available

References

• Original: J:333861 Qin J, et al., Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation. Cell Death Differ. 2023 Jan;30(1):184-194
• All: 1 reference(s)