Hoxd11^em1Dmwe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hoxd11^em1Dmwe
• Name: homeobox D11; endonuclease-mediated mutation 1, Deneen M Wellik
• MGI ID: MGI:8282413
• Synonyms: Hoxd11-3XFLAG, Hoxd11^FL
• Gene: Hoxd11 Location: Chr2:74509902-74517360 bp, + strand Genetic Position: Chr2, 44.13 cM
• Alliance: Hoxd11^em1Dmwe page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology inserted a 3X FLAG tag at the 5 end, nine base pairs downstream form the translational start site (ATG) in-frame with the coding sequence. PCR genotyping confirmed the correct size for the 3X FLAG insertion and correct in-frame targeting was assessed by sequencing. (J:367276)

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxd11 Mutation: 14 strains or lines available

References

• Original: J:367276 Echevarria-Andino ML, et al., Generation of Hoxa11-3XFLAG and Hoxd11-3XFLAG alleles to investigate Hox11 genome-wide binding. Dev Biol. 2025 May 17;524:210-218
• All: 1 reference(s)