Emcn^tm1.2Pdmr
Targeted Allele Detail

Summary

• Symbol: Emcn^tm1.2Pdmr
• Name: endomucin; targeted mutation 1.2, Patricia D'Amore
• MGI ID: MGI:8278585
• Gene: Emcn Location: Chr3:137046824-137136830 bp, + strand Genetic Position: Chr3, 63.65 cM
• Alliance: Emcn^tm1.2Pdmr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:366307
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-11 were replaced with a loxP site, FRT-flanked neomycin resistance gene, exons 2 to 10, an F3-flanked neomycin resistance gene, lox2272 site, a promotor-less reporter EGFP in reverse orientation, loxP site and lox2272 site. The neomycin resistance genes were removed via flp-mediated recombination. Cre-mediated recombination deleted exons 2 to 10 and inverted the EGFP. Western blot analysis confirmed the absence of protein in kidney, lung and thyroid gland of homozygotes. (J:366307)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Emcn Mutation: 28 strains or lines available

References

• Original: J:366307 Hu Z, et al., Loss of the Endothelial Glycocalyx Component EMCN Leads to Glomerular Impairment. Circ Res. 2025 Jan 3;136(1):59-74
• All: 1 reference(s)