Prkaa2^em1Smz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prkaa2^em1Smz
• Name: protein kinase, AMP-activated, alpha 2 catalytic subunit; endonuclease-mediated mutation 1, Shi-Min Zhao
• MGI ID: MGI:8276809
• Synonyms: alpha2^T172A
• Gene: Prkaa2 Location: Chr4:104887071-104967087 bp, - strand Genetic Position: Chr4, 49.05 cM
• Alliance: Prkaa2^em1Smz page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: Threonine codon 172 (ACT) was changed to alanine (GCT) (p.T172A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. In the encoded protein, the mutation replaces a phosphorylatable residue with one that cannot, which reduces its protein synthesis inhibitory activity, leading to the development of mild Alzheimers disease (AD) symptoms. (J:377422)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkaa2 Mutation: 33 strains or lines available

References

• Original: J:377422 Mao Y, et al., AMPKalpha2 signals amino acid insufficiency to inhibit protein synthesis. Cell Metab. 2026 Jan 6;38(1):192-207.e8
• All: 1 reference(s)