Caprin2^em1Nshi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Caprin2^em1Nshi
• Name: caprin family member 2; endonuclease-mediated mutation 1, Nobuyuki Shiina
• MGI ID: MGI:8254854
• Synonyms: Rng140^-
• Gene: Caprin2 Location: Chr6:148743990-148797735 bp, - strand Genetic Position: Chr6, 78.62 cM
• Alliance: Caprin2^em1Nshi page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting of exon 6 deleted 5 bp. This deletion caused a frameshift in the Caprin2 ORF and generated a downstream premature stop codon in exon 7. The premature stop codon in the 7th exon of a total of 19 exons was shown to cause non-sense mediated decay of mRNA, resulting in much less expression of the truncated form. Western blot analysis showed that protein expression is significantly decreased in the eyes of homozygous mutant mice at P0.5. Immunostaining of P0.5 eye slices with an anti-CAPRIN2 antibody revealed a decrease in lens fiber cell staining. (J:299943)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Caprin2 Mutation: 36 strains or lines available

References

• Original: J:299943 Nakazawa K, et al., Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation. J Biol Chem. 2020 Oct 30;295(44):15029-15044
• All: 1 reference(s)