Igs2^{em2(Rr29-mCherry,EGFP)Ezk}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em2(Rr29-mCherry,EGFP)Ezk}
• Name: intergenic site 2; endonuclease-mediated mutation 2, Evgeny Z Kvon
• MGI ID: MGI:8240190
• Synonyms: ZRS-mCherry/Empty-eGFP
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em2(Rr29-mCherry,EGFP)Ezk} page

Mutation origin

• Strain of Origin: FVB/NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: The transgene, inserted into chr11, contains the following elements: long-range Shh enhancer Rr29 (ZRS) upstream of mouse Hspa1 minimal promoter, mCherry reporter gene cDNA and SV40 poly(A) signal sequences (all in reverse orientation), a synthetic strong insulator, mouse Hspa1 minimal promoter, EGFP reporter gene cDNA and SV40 poly(A) signal sequences. Through short range activity of the enhancer, the allele expresses only mCherry in the embryonic limb buds; the insulator blocks the enhancer from controlling EGFP expression. (J:370789)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 66 strains or lines available

References

• Original: J:370789 Bower G, et al., Range extender mediates long-distance enhancer activity. Nature. 2025 Jul;643(8072):830-838
• All: 1 reference(s)