C3^tm2(C3)Lgmo
Targeted Allele Detail

Summary

• Symbol: C3^tm2(C3)Lgmo
• Name: complement component 3; targeted mutation 2, Lori G Morton
• MGI ID: MGI:8224027
• Synonyms: C3^hu
• Gene: C3 Location: Chr17:57510970-57535136 bp, - strand Genetic Position: Chr17, 29.72 cM, cytoband E1-E3
• Alliance: C3^tm2(C3)Lgmo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:369997
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• C3^tm2(C3)Lgmo expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  C3 (718)

  C3	(MGI:88227)

• Mutation details: Using VelociGene technology, a targeting construct consisting of human C3 sequence extending from 9-kb upstream of coding exon 1 to 1.5-kb downstream of the polyA signal, followed by a loxP-hyg-loxP cassette was electroporated into C3^tm1Lgmo ES cells, resulting in the replacement of the floxed neomycin cassette (which replaced 30.6 kb of the mouse sequence including 6.6 kb of 5 regulatory region and exons 1-41), with the human C3 sequence. The inserted human C3 contains the CGC (arginine) variant C3S. Serum mouse C3 protein levels are absent in homozygous and human C3 protein levels are about half that of the mouse C3 levels seen in wild-type mice. (J:369997)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C3 Mutation: 100 strains or lines available

Notes

The allele was generated in 129 x C57BL/6 ES cells carrying C3^tm1Lgmo. (J:369997)

References

• Original: J:369997 Devalaraja-Narashimha K, et al., Humanized C3 Mouse: A Novel Accelerated Model of C3 Glomerulopathy. J Am Soc Nephrol. 2021 Jan;32(1):99-114
• All: 1 reference(s)