Gt(ROSA)26Sor^{em2(CAG-tdTomato*,-cre,-EYFP*)Ben}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-tdTomato*,-cre,-EYFP*)Ben}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Rui Benedito
• MGI ID: MGI:8192456
• Synonyms: FlpMosaics, R26-iFlp^{MTomato-2A-Cre/MYFP}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-tdTomato*,-cre,-EYFP*)Ben} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:366224
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Recombinase, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em2(CAG-tdTomato*,-cre,-EYFP*)Ben} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: CRISPR-targeting deleted 2A-FlpO from Gt(ROSA)26Sor^{em1(CAG-tdTomato*,-cre,-EYFP, flpo*)Ben}. (J:366224)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1097 strains or lines available

References

• Original: J:366224 Garcia-Gonzalez I, et al., iFlpMosaics enable the multispectral barcoding and high-throughput comparative analysis of mutant and wild-type cells. Nat Methods. 2025 Feb;22(2):323-334
• All: 1 reference(s)