Mblac1^em1Rbl
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mblac1^em1Rbl
• Name: metallo-beta-lactamase domain containing 1; endonuclease-mediated mutation 1, Randy D Blakely
• MGI ID: MGI:8186384
• Synonyms: Mblac1 KO
• Gene: Mblac1 Location: Chr5:138192576-138193883 bp, + strand Genetic Position: Chr5, 76.98 cM
• Alliance: Mblac1^em1Rbl page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A CRISPR/Cas9 based strategy generated a 5 bp deletion in exon 1, leading to a frameshift that results in the generation of 27 amino acids of ectopic sequence prior to strand termination. Immunoblot analysis of brain (cortical tissue) and liver extracts confirmed the absence of protein expression in homozygous mutant mice. (J:344443)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mblac1 Mutation: 8 strains or lines available

References

• Original: J:344443 Gibson CL, et al., Global untargeted serum metabolomic analyses nominate metabolic pathways responsive to loss of expression of the orphan metallo beta-lactamase, MBLAC1. Mol Omics. 2018 Jun 12;14(3):142-155
• All: 2 reference(s)