Ncs1^m1Rbrc
Chemically induced Allele Detail

Summary

• Symbol: Ncs1^m1Rbrc
• Name: neuronal calcium sensor 1; mutation 1, RIKEN BioResource Center
• MGI ID: MGI:8162228
• Synonyms: Ncs1^P144S
• Gene: Ncs1 Location: Chr2:31135835-31186001 bp, + strand Genetic Position: Chr2, 21.81 cM, cytoband B
• Alliance: Ncs1^m1Rbrc page

Mutation origin

• Strain of Origin: (DBA/2 x C57BL/6)F1

Mutation description

• Allele Type: Chemically induced (ENU) (Hypomorph)
• Mutation: Single point mutation
• Mutation details: ENU mutagenesis was used to induce a C to T mutation that is located within exon 6 at position 2:31,284,698 (ENSMUSG00000062661) and changes the proline at position 144 to a serine (P144S) in a region between the third and fourth EF-hand of the encoded protein. This point mutation introduces a third degree of freedom of rotation at residue 144 and potential for at least two additional hydrogen bonds that could interfere with proper protein folding and/or give rise to protein instability. Quantitative PCR showed no differences in whole brain mRNA levels whereas immunoblot analysis revealed a gene dose-dependent reduction in protein levels, consistent with the in-silico predicted destabilization of protein structure. Use of a different antibody confirmed the significant reduction of protein expression in hippocampal lysates from homozygous mutant mice. (J:251103)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncs1 Mutation: 15 strains or lines available

References

• Original: J:251103 Mun HS, et al., Self-directed exploration provides a Ncs1-dependent learning bonus. Sci Rep. 2015 Dec 7;5:17697
• All: 1 reference(s)