Igs2^{em1(CAG-RFP*/GFP*)Qsun}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em1(CAG-RFP*/GFP*)Qsun}
• Name: intergenic site 2; endonuclease-mediated mutation 1, Qiming Sun
• MGI ID: MGI:7865173
• Synonyms: CA-ER-TRG
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em1(CAG-RFP*/GFP*)Qsun} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details:

  Using CRISPR-Cas9 gene editing, a CAG promoter cassette, a loxP-flanked STOP cassette containing polyA sequences, and the open reading frame (ORF) for an N-terminal ER signal sequence followed by tandem monomeric RFP and GFP sequences and the ER retention sequence KDEL (the ssRFP-GFPKDEL fusion protein), including a Kozak sequence (GCCACC), were inserted into the Igs2 (H11) locus of the mouse genome. The Cre recombinase activates the expression of ssRFP-GFPKDEL. (J:361749)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 54 strains or lines available

References

• Original: J:361749 Sang Y, et al., Visualizing ER-phagy and ER architecture in vivo. J Cell Biol. 2024 Dec 2;223(12)
• All: 1 reference(s)