Prg2^{tm2(cre/ERT2)Ics}
Targeted Allele Detail

Summary

• Symbol: Prg2^{tm2(cre/ERT2)Ics}
• Name: proteoglycan 2, bone marrow; targeted mutation 2, Mouse Clinical Institute
• MGI ID: MGI:7863605
• Gene: Prg2 Location: Chr2:84810805-84813976 bp, + strand Genetic Position: Chr2, 49.45 cM
• Alliance: Prg2^{tm2(cre/ERT2)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Prg2^{tm2(cre/ERT2)Ics} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prg2 (MGI:103294)

• Mutation details: A 5.6 kb trap cassette was inserted by homologous recombination in ES cells in the intronic region of Prg2 located between exon 2 and exon 3. This cassette contains intron 1 and partial exon sequence of En2 to act as splice donor site, a LF2A (2A self-cleaving peptide) sequence that can induce ribosomal skipping during translation, a mutated TagRFP657 far-red monomeric fluorescent protein sequence, a T2A sequence and a dual cre-F3-ERT2-F3 cassette that allow expression of creERT2 or cre (if crossed with a flp deleter) and a selection cassette. The pPGK-Neo selection cassette flanked by rox attachment sites was not removed. (J:82809)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prg2 (mouse) Summary of all recombinase alleles driven by Prg2. Alliance mouse Prg2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prg2 Mutation: 22 strains or lines available

Notes

ES cell = BA3 (BALB/cN)

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)