Trac^{em2(Tcrb,Tcra)Strom}
Allele Detail

Summary

• Symbol: Trac^{em2(Tcrb,Tcra)Strom}
• Name: T cell receptor alpha constant; endonuclease-mediated mutation 2, Ingunn Stromnes
• MGI ID: MGI:7645419
• Synonyms: 7431 TRex
• Gene: Trac Location: Chr14:54457978-54461655 bp, + strand Genetic Position: Chr14, 27.7 cM
• Alliance: Trac^{em2(Tcrb,Tcra)Strom} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Not Applicable (Inserted expressed sequence, Null/knockout)
• Mutations: Deletion, Intragenic deletion
• Trac^{em2(Tcrb,Tcra)Strom} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: CRISPR technology was used to introduce an in-frame, low affinity (clone 7431), codon-optimized, Msln406-414:H-2Db-specific Valpha4/Vbeta9 T cell receptor (TCR) clone 7431 consisting of TCRb variable (V), TCRb constant (C) and TCRa V, linked by a self-cleaving P2A element to exon 1 of the mouse Trac gene and simultaneously delete 23 bp from the 5' end of exon 4 of the endogenous Msln gene to create a knock-out allele. (J:333043)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trac Mutation: 8 strains or lines available

References

• Original: J:333043 Rollins MR, et al., Germline T cell receptor exchange results in physiological T cell development and function. Nat Commun. 2023 Feb 1;14(1):528
• All: 1 reference(s)