Actb^{em3(tetO-GRK2*/mCherry)Kta}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actb^{em3(tetO-GRK2*/mCherry)Kta}
• Name: actin, beta; endonuclease-mediated mutation 3, Kohichi Tanaka
• MGI ID: MGI:7610683
• Synonyms: Actb^{em3(tetO-FLEX-ibARK/mCherry)Kta}
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{em3(tetO-GRK2*/mCherry)Kta} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Actb^{em3(tetO-GRK2*/mCherry)Kta} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Actb (MGI:87904)

• Mutation details: CRISPR-targeting inserted the Escherichia coli tetO, cytomegalovirus CMV minimal promoter, rabbit Beta globin intron, bacteriophage loxP, lox2272, bovine GRK2, porcine technovirus 1 P2A, human GH polyA, Discosoma sp. mCherry, Woodchuck hepatitis virus WPRE, chemical synthesis DNA FLAG tag, human influenza virus HA tag, Streptococcus pyogenes crRNA, tracrRNA (gRNA). (J:104881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 55 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 1 reference(s)