Actb^{em1(tetO-EGFP)Kta}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actb^{em1(tetO-EGFP)Kta}
• Name: actin, beta; endonuclease-mediated mutation 1, Kohichi Tanaka
• MGI ID: MGI:7610673
• Synonyms: Actb-tetO-FLEX-EGFP
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{em1(tetO-EGFP)Kta} page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted a tetracycline response element fused to a minimal cytomegalovirus (CMV) promoter driving inducible and conditional expression of inverted EGFP flanked by two pairs of loxP and lox2722 (FLEX switch) and polyadenylation sequence. (J:345651)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 55 strains or lines available

References

• Original: J:345651 Aida T, et al., Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice. Genome Biol. 2015 Apr 29;16:87
• All: 2 reference(s)