Fcgr1^{em1(FCGR1A)Synbl}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fcgr1^{em1(FCGR1A)Synbl}
• Name: Fc receptor, IgG, high affinity I; endonuclease-mediated mutation 1, Synbal
• MGI ID: MGI:7607010
• Synonyms: Humanized Mouse-hFcgr1
• Gene: Fcgr1 Location: Chr3:96190225-96201285 bp, - strand Genetic Position: Chr3, 41.72 cM
• Alliance: Fcgr1^{em1(FCGR1A)Synbl} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Fcgr1^{em1(FCGR1A)Synbl} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FCGR1A (2209)

  Fcgr1

  (MGI:95498)

• Mutation details: Using a CRISPR/Cas9 approach, an active genetic cassette (AGC) was used to remove the endogenous mouse gene coding region and replace it with a functional human FCGR1A coding region under the control of the endogenous mouse promoter. The AGC included a Fcgr1 5HR, Fcgr1 Exon1-Start-P2A sequence, human FCGR1A mini gene, hFCGR1A 3UTR, U6 promoter, gRNA, Stop-FRT cassette, mFcgR1A Exon-1, mFcgR1A 3HR. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fcgr1 Mutation: 34 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)