Trac^em2Gmrs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Trac^em2Gmrs
• Name: T cell receptor alpha constant; endonuclease-mediated mutation 2, Gerald P Morris
• MGI ID: MGI:7576105
• Synonyms: TCRA-RFP
• Gene: Trac Location: Chr14:54457978-54461655 bp, + strand Genetic Position: Chr14, 27.7 cM
• Alliance: Trac^em2Gmrs page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, a red fluorescent protein (tdTomato) sequence at the 3' end of exon 3, at the C-terminal end of the T cell receptor alpha constant (Trac) gene attached by an 18-amino-acid flexible linker protein (GSGSGSGSGSGSGSQPVAT). (J:301323)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trac Mutation: 8 strains or lines available

References

• Original: J:301323 Yang L, et al., TCRalpha reporter mice reveal contribution of dual TCRalpha expression to T cell repertoire and function. Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32574-32583
• All: 1 reference(s)