Lrwd1^tm1Kmcn
Targeted Allele Detail

Summary

• Symbol: Lrwd1^tm1Kmcn
• Name: leucine-rich repeats and WD repeat domain containing 1; targeted mutation 1, Kui Ming Chan
• MGI ID: MGI:7574465
• Synonyms: Lrwd1^-, Lrwd1^neo
• Gene: Lrwd1 Location: Chr5:136151920-136164928 bp, - strand Genetic Position: Chr5, 75.8 cM
• Alliance: Lrwd1^tm1Kmcn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326014
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced an FRT-flanked beta-neomycin resistance cassette with a 3' loxP site into intron 5. An additional loxP site was inserted downstream of exon 7. The gene trap disrupted gene expression downstream of the linker region and abrogated the expression of the WD40 region which associates directly with the origin recognition complex (ORC) and repressive histone marks. RT-qPCR analysis of primary mouse embryonic fibroblasts (MEFs) from E13.5 homozygous embryos showed complete disruption of transcription from exon 6. Western blot analysis using a LRWD1 antibody recognizing the linker region confirmed loss of protein expression in homozygous MEFs. (J:326014)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lrwd1 Mutation: 36 strains or lines available

References

• Original: J:326014 Kang TZE, et al., Lrwd1 impacts cell proliferation and the silencing of repetitive DNA elements. Genesis. 2022 May;60(4-5):e23475
• All: 1 reference(s)