Igs2^{em1.1(CAG-cas12a*)Mmw}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igs2^{em1.1(CAG-cas12a*)Mmw}
• Name: intergenic site 2; endonuclease-mediated mutation 1.1, Monte Winslow
• MGI ID: MGI:7574256
• Synonyms: H11^Cas12a
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{em1.1(CAG-cas12a*)Mmw} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector contains a CMV enhancer/chicken beta-actin core promoter (CAGGS), a loxP-STOP(6xSV40 polyA)-loxP cassette (LSL), an enhanced Acidaminococcus cas12a variant, including several substitutions (E174R/N282A/S542R/K548R; enAsCas12a), a nucleoplasmin nuclear localization signal (NLS), a 3xHA epitope sequence, an SV40 NLS and a rabbit beta-globin polyadenylation signal (RBG pA). Cre-mediated recombination removed the STOP cassette. (J:101977, J:367787)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:367787 Hebert JD, et al., Efficient and multiplexed somatic genome editing with Cas12a mice. Nat Biomed Eng. 2025 May 30
• All: 2 reference(s)