Malt1^em1Krad
Endonuclease-mediated Allele Detail

Summary

• Symbol: Malt1^em1Krad
• Name: MALT1 paracaspase; endonuclease-mediated mutation 1, Daniel Krappmann
• MGI ID: MGI:7571552
• Synonyms: Malt1^TBM
• Gene: Malt1 Location: Chr18:65564010-65611959 bp, + strand Genetic Position: Chr18, 38.56 cM
• Alliance: Malt1^em1Krad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333424
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Endonuclease-mediated (Constitutively active)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated an A to C change resulting in a glutamate to alanine substitution at amino acid 325 (E325A) in the TRAF6 biding motif 1 (T6BM1) in exon 7, an A to C change resulting in a glutamate to alanine substitution at codon 814 (E814A) in the TRAF6 biding motif 2 (T6BM2) in exon 17 of the Malt1a splice variant, and an A to C change resulting in a glutamate to alanine substitution at amino acid 803 (p.E803A) in the T6BM2 of the Malt1b splice variant. These missense mutations render MALT1 incapable of interacting with TRAF6. Differential PCR and genomic and complementary DNA sequencing verified correct genome editing and Western blot analysis showed equivalent protein expression in heterozygous and homozygous T cells as in controls. Loss of TRAF6 biding causes constitutive MALT1 protease activation. (J:333424)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Malt1 Mutation: 33 strains or lines available

Notes

ES cell line = R1/E (J:333424)

References

• Original: J:333424 O'Neill TJ, et al., TRAF6 prevents fatal inflammation by homeostatic suppression of MALT1 protease. Sci Immunol. 2021 Nov 12;6(65):eabh2095
• All: 1 reference(s)