Igs2^{tm1(RNU6-gRNA:Top1,-mCherry)Jtu}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(RNU6-gRNA:Top1,-mCherry)Jtu}
• Name: intergenic site 2; targeted mutation 1, James Turner
• MGI ID: MGI:7561602
• Synonyms: H11^Top1
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(RNU6-gRNA:Top1,-mCherry)Jtu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316814
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Knockdown, Reporter)
• Mutation: Insertion
• Mutation details: Integrase targeting introduced the human U6 driving expression of sgRNA2 targeting the DNA binding domain of Topoisomerase 1, and promoterless mCherry reporter gene into the locus. (J:316814)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:316814 Douglas C, et al., CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes. Nat Commun. 2021 Dec 3;12(1):6926
• All: 1 reference(s)