Tbl1xr1^tm1.1Meln
Targeted Allele Detail

Summary

• Symbol: Tbl1xr1^tm1.1Meln
• Name: transducin (beta)-like 1X-linked receptor 1; targeted mutation 1.1, Ari Melnick
• MGI ID: MGI:7550437
• Synonyms: Tbl1xr1^D370Y
• Gene: Tbl1xr1 Location: Chr3:22130816-22270758 bp, + strand Genetic Position: Chr3, 7.58 cM
• Alliance: Tbl1xr1^tm1.1Meln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292118
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 10 and a lox66 site, duplicates of exon 11 and 12, where aspartic acid codon 370 (GAC) in exon 12 was changed to tyrosine (TAC) (p.D370Y), and a lox71 site were inserted in reverse orientation into intron 12. An FRT site flanked neomycin resistance gene cassette was inserted between endogenous exon 12 and the inserted duplicate exons. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses wildtype transcripts/peptide. Only after conditional Cre-mediated inversion of the inserted duplicate exons (with mutated exon 12) and deletion of the endogenous exons 11 and 12 will it express mutated transcript/peptide. (J:292118)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbl1xr1 Mutation: 322 strains or lines available

References

• Original: J:292118 Venturutti L, et al., TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate. Cell. 2020 Jul 23;182(2):297-316.e27
• All: 1 reference(s)