Vcan^em1Hwat
Endonuclease-mediated Allele Detail

Summary

• Symbol: Vcan^em1Hwat
• Name: versican; endonuclease-mediated mutation 1, Hideto Watanabe
• MGI ID: MGI:7548796
• Synonyms: V1R
• Gene: Vcan Location: Chr13:89803431-89890628 bp, - strand Genetic Position: Chr13, 45.5 cM
• Alliance: Vcan^em1Hwat page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Alanine codon 1402 (in ENSMUST00000109546) and arginine codons 1403 and 1404 (GCTAGGCGT) were changed to asparagine-valine-tyrosine (AACGTGTAC) (A442_R444delinsNVY) using a crRNA (GAAGCUGCAGAAGCUAGGCGguuuuagagcuaugcuguuuug), a tracrRNA and an ssODN template with CRISPR/Cas9 technology. The mutation, near the ADAMTS cleavage site in the encoded peptide, blocks cleavage. (J:298045)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vcan Mutation: 164 strains or lines available

References

• Original: J:298045 Islam S, et al., Accumulation of versican facilitates wound healing: Implication of its initial ADAMTS-cleavage site. Matrix Biol. 2020 May;87:77-93
• All: 2 reference(s)