Scyl1^tm2.1Spel
Endonuclease-mediated Allele Detail

Summary

• Symbol: Scyl1^tm2.1Spel
• Name: SCY1-like 1 (S. cerevisiae); targeted mutation 2.1, Stephane Pelletier
• MGI ID: MGI:7545664
• Synonyms: Scyl1^AIv4delta
• Gene: Scyl1 Location: Chr19:5808450-5821461 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Scyl1^tm2.1Spel page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: CRISPR-targeting of exon 3 inserted an Artificial Intron version 4 (AIv4) encoding a splice donor, essential intronic sequences flanked by loxP sites, and a splice acceptor site. Cre-mediated recombination removed the branch point and part of the polypyrimidine tract leaving a string of stop codons in all three frames. (J:342085)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scyl1 Mutation: 21 strains or lines available

References

• Original: J:342085 Cassidy AM, et al., One-step generation of a conditional allele in mice using a short artificial intron. Heliyon. 2022;8(12):e12630
• All: 1 reference(s)