Xist^tm7Sado
Targeted Allele Detail

Summary

• Symbol: Xist^tm7Sado
• Name: inactive X specific transcripts; targeted mutation 7, Takashi Sado
• MGI ID: MGI:7539731
• Synonyms: Xist^CAGdelta5'2L
• Gene: Xist Location: ChrX:102503979-102526839 bp, - strand Genetic Position: ChrX, 46.15 cM, cytoband D
• Alliance: Xist^tm7Sado page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243417
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A CAG promoter and a loxP site flanked puromycin resistance gene and ECFP reporter gene double cassette were inserted upstream of exon 1 replacing the native promoter and the 5UTR. This conditional-ready allele expresses the selection and reporter genes but not the targeted gene. (J:243417)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Xist Mutation: 11 strains or lines available

References

• Original: J:243417 Sakata Y, et al., Defects in dosage compensation impact global gene regulation in the mouse trophoblast. Development. 2017 Aug 01;144(15):2784-2797
• All: 1 reference(s)