Igs2^{tm3(CAG-CaMBI300)Mzlin}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm3(CAG-CaMBI300)Mzlin}
• Name: intergenic site 2; targeted mutation 3, Michael Z Lin
• MGI ID: MGI:7536914
• Synonyms: CAG-LSL-CaMBI-300, Orange CaMBI 300
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm3(CAG-CaMBI300)Mzlin} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341258
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The allele is created using site-specific PhiC31 integrase-mediated transgenesis to insert a loxP-flanked stop cassette, and the Antares-derived, orange-emitting, calcium-modulated bioluminescent indicator (CaMBI) followed by a polyA signal and directed by a chicken beta-actin promoter with a CMV enhancer (CAG promoter). CamBI 300 is composed of CyOFP1::N-terminal NLuc::1aa linker (I)::CaM::1aa linker (G)::M13::2aa linker (EL)::C-terminal NLuc::CyOFP1. (J:341258)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:341258 Oh Y, et al., An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging. Nat Chem Biol. 2019 May;15(5):433-436
• All: 1 reference(s)