Igs2^{tm2(CAG-NanoLuc/CyOFP1)Mzlin}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm2(CAG-NanoLuc/CyOFP1)Mzlin}
• Name: intergenic site 2; targeted mutation 2, MIchael Z Lin
• MGI ID: MGI:7532826
• Synonyms: H11P-CAG-LSL-Antares
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm2(CAG-NanoLuc/CyOFP1)Mzlin} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341167
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The allele is created using site-specific PhiC31 integrase-mediated transgenesis to insert a loxP-flanked stop cassette, a Kozak sequence and the CyOFP1-NanoLuc-CyOFP1 fusion bioluminescent reporter (Antares) directed by a chicken beta-actin promoter with a CMV enhancer (CAG promoter). Antares is composed of the highly-catalytic, blue-emitting firefly luciferase derivative NanoLuc fused to two copies of the fluorescent protein CyOFP1. CyOFP1 is a basic (constitutively fluorescent) long stokes shift fluorescent protein derived from Entacmaea quadricolor. (J:341167)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

References

• Original: J:341167 Su Y, et al., Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals. Nat Methods. 2020 Aug;17(8):852-860
• All: 2 reference(s)