Dlgap3^tm2.1Gfng
Targeted Allele Detail

Summary

• Symbol: Dlgap3^tm2.1Gfng
• Name: DLG associated protein 3; targeted mutation 2.1, Guoping Feng
• MGI ID: MGI:7523891
• Synonyms: SAPAP3 cKI
• Gene: Dlgap3 Location: Chr4:127062997-127130815 bp, + strand Genetic Position: Chr4, 61.33 cM
• Alliance: Dlgap3^tm2.1Gfng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339639
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: The targeted allele is created to have an inverted exons 8-10 ((Ensemble ID: ENSMUSE00001223455, ENSMUSE00000400411, ENSMUSE00000335250, and introns) flanked with the FLEx cassette (inward-facing lox2272:loxP sites), followed by a FRT-flanked neomycin resistance cassette. The inversion creates a reading frame shift resulting in a null allele. Flp-mediated recombination removes the neo cassette. (J:339639)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dlgap3 Mutation: 83 strains or lines available

References

• Original: J:339639 Malgady JM, et al., Non-synaptic alterations in striatal excitability and cholinergic modulation in a SAPAP3 mouse model of compulsive motor behavior. bioRxiv. 2022
• All: 2 reference(s)