Gt(ROSA)26Sorem3(CAG-Gf1i,-Atoh1/EGFP,-Pou4f3)Akg
Endonuclease-mediated Allele Detail
|
Symbol: |
Gt(ROSA)26Sorem3(CAG-Gf1i,-Atoh1/EGFP,-Pou4f3)Akg |
Name: |
gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 3, Andrew K Groves |
MGI ID: |
MGI:7522011 |
Synonyms: |
Rosa26-GAP |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
|
Alliance: |
Gt(ROSA)26Sorem3(CAG-Gf1i,-Atoh1/EGFP,-Pou4f3)Akg page
|
|
|
Allele Type: |
|
Endonuclease-mediated (Conditional ready, Inserted expressed sequence, Reporter) |
Mutation: |
|
Insertion
|
|
|
Gt(ROSA)26Sorem3(CAG-Gf1i,-Atoh1/EGFP,-Pou4f3)Akg expresses
3 genes
|
|
|
Mutation details: The targeting vector is designed using a modified Ai3 (Gt(ROSA)26So) vector where the eGFP sequence was replaced with coding regions for growth factor independent 1 transcription repressor (Gfi1), atonal bHLH transcription factor 1 (Atoh1) fused to an enhanced green fluorescent protein (eGFP) and POU domain, class 4, transcription factor 3 (Pou4f3). Specifically, the targeting vector includes (from 5' to 3') a splice acceptor, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP (with stop codons in all 3 reading frames and a triple polyA signal) cassette, Gfi1 sequence, a GSG oligopeptide linker-P2A peptide sequence, Atoh1/eGFP fusion gene, a second GSG oligopeptide linker-P2A peptide sequence, Pou4f3 sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), polyA sequence, followed by an attB/attP-flanked PGK-neo-PGKpA cassette.
This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
(J:339290)
|
|
|
|
Original: |
J:339290 Iyer AA, et al., Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals. Elife. 2022 Nov 29;11 |
All: |
1 reference(s) |
|