Fus^tm2.1Neas
Targeted Allele Detail

Summary

• Symbol: Fus^tm2.1Neas
• Name: fused in sarcoma; targeted mutation 2.1, Neil A Shneider
• MGI ID: MGI:7510137
• Synonyms: FUS C14
• Gene: Fus Location: Chr7:127565276-127581204 bp, + strand Genetic Position: Chr7, 69.87 cM
• Alliance: Fus^tm2.1Neas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326930
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, No functional change)
• Mutation: Insertion
• Mutation details: Exon 14 was flanked by loxP sites and part of exon 15 was replaced with a STOP cassette and human exon 15 sequence. The FRT-flanked neomycin resistance cassette that was inserted 50 bp downstream of the end of the 3 UTR was removed via flp-mediated recombination. This allele produces wild-type mouse protein. However, cre-mediated recombination can be used to remove exon 14 and modify the allele to reproduce an amyotrophic lateral sclerosis-associated human mutation (p.G466VfsX14) which leads to a reading frameshift and incorporation of 14 miscoded amino acids from exon 15 before an early termination codon and a C-terminal truncation. (J:326930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fus Mutation: 57 strains or lines available

Notes

GFP-expressing LB10 mouse embryonic stem cells derived from a C57BL6/N mouse.

References

• Original: J:326930 Korobeynikov VA, et al., Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis. Nat Med. 2022 Jan;28(1):104-116
• All: 1 reference(s)