Abtb3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Abtb3^{tm1c(EUCOMM)Wtsi}
• Name: ankyrin repeat and BTB domain containing 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7493862
• Synonyms: Btbd11^F
• Gene: Abtb3 Location: Chr10:85222678-85496156 bp, + strand Genetic Position: Chr10, 41.65 cM, cytoband C1
• Alliance: Abtb3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Abtb3 gene page

Mutation origin

• Mutant Cell Line: EPD0463_1_A11
• Germline Transmission: Earliest citation of germline transmission: J:337062
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85460286 of Chromosome 10 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 85461263. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:337062)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abtb3 Mutation: 151 strains or lines available

References

• Original: J:337062 Bygrave AM, et al., Btbd11 supports cell-type-specific synaptic function. Cell Rep. 2023 May 31;42(6):112591
• All: 1 reference(s)