Gt(ROSA)26Sor^{tm1(CAG-Gria2*)Bli}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Gria2*)Bli}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bo Li
• MGI ID: MGI:7491788
• Synonyms: Rosa26-stop^flox-GluA2^mut
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Gria2*)Bli} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262751
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm1(CAG-Gria2*)Bli} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gria2 (MGI:95809)	contains L483Y and R845A mutations

• Mutation details: The targeting cassette includes (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), Gria2 mutant cDNA (containing two point mutations, L483Y and R845A), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This targeting vector was inserted into intron 1 of the Gt(ROSA)26Sor locus. The L483Y mutation impairs AMPA receptor desensitization and the R845A mutation blocks AMPA receptor endocytosis. (J:262751)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

References

• Original: J:262751 Ahrens S, et al., A Central Extended Amygdala Circuit That Modulates Anxiety. J Neurosci. 2018 Jun 13;38(24):5567-5583
• All: 1 reference(s)