Esm1^tm1.1Rha
Targeted Allele Detail

Summary

• Symbol: Esm1^tm1.1Rha
• Name: endothelial cell-specific molecule 1; targeted mutation 1.1, Ralf H Adams
• MGI ID: MGI:7490359
• Synonyms: Esm1^KO
• Gene: Esm1 Location: Chr13:113346193-113354632 bp, + strand Genetic Position: Chr13, 63.93 cM
• Alliance: Esm1^tm1.1Rha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246778
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP recognition sites were inserted around the second exon of the murine Esm1 gene via homologous recombination in the ES cells. A murine cDNA fragment containing the coding sequence from exons 2 and 3 followed by 2 copies of the bovine growth hormone polyadenylation signal sequence was inserted in frame, and downstream of the loxP-flanked area, the targeting construct also contained a beta-galactosidase (lacZ) reporter cassette, which carried an artificial splice acceptor and an internal ribosomal entry site (IRES) at the 5-end and the SV40 polyadenylation signal sequence at the 3-end. The targeting construct also contained a neomycin resistance cassette surrounded by Frt sites allowing Flp recombinase-mediated removal. Through interbreeding with PGK-Cre deleter mice, exon 2 of the Esm1 gene was globally deleted. No beta-galactosidase reporter activity was detectable in heterozygous or homozygous mutants, and it was subsequently confirmed that Esm1-lacZ fusion transcripts were not detectable by qPCR. (J:246778)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Esm1 Mutation: 18 strains or lines available

References

• Original: J:246778 Rocha SF, et al., Esm1 modulates endothelial tip cell behavior and vascular permeability by enhancing VEGF bioavailability. Circ Res. 2014 Aug 29;115(6):581-90
• All: 2 reference(s)