Rorc^em1Wjmh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rorc^em1Wjmh
• Name: RAR-related orphan receptor gamma; endonuclease-mediated mutation 1, Wendy Jia Men Huang
• MGI ID: MGI:7488172
• Synonyms: RORgammat^S182A
• Gene: Rorc Location: Chr3:94280106-94305583 bp, + strand Genetic Position: Chr3, 40.56 cM, cytoband F2
• Alliance: Rorc^em1Wjmh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: Serine codon 182 (AGT) (in reference to the shorter t isoform) was changed to alanine (GCT) (p.S182A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This mutation prevents phosphorylation of the residue in the encoded peptide. (J:324904)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rorc Mutation: 42 strains or lines available

References

• Original: J:324904 Ma S, et al., RORgammat phosphorylation protects against T cell-mediated inflammation. Cell Rep. 2022 Mar 15;38(11):110520
• All: 1 reference(s)