Actb^{tm1.1(tetO-GFP,-pac)Yksk}
Targeted Allele Detail

Summary

• Symbol: Actb^{tm1.1(tetO-GFP,-pac)Yksk}
• Name: actin, beta; targeted mutation 1.1, Yuji Ikegaya
• MGI ID: MGI:7471128
• Synonyms: Actb^{tm1.1(tetO-GFP/bPAC)Yksk}, tetO-GFP-2A-bPAC
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{tm1.1(tetO-GFP,-pac)Yksk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300732
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Reporter)
• Mutation: Insertion
• Mutation details: The targeting contruct inserted into the 3'UTR an FRT-flanked neomycin resistance cassette, rabbit beta globin intron, tetracycline-inducible promoter driving expression of the GFP reporter gene, P2A sequence, and photoactivated cyclase from Beggiatoa (pac). Flp-mediated recombination removed the selection cassette. (J:300732)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 55 strains or lines available

References

• Original: J:300732 Zhou Z, et al., Astrocytic cAMP modulates memory via synaptic plasticity. Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):e2016584118
• All: 4 reference(s)