Igs2^{tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu}
• Name: intergenic site 2; targeted mutation 1, Ming Xu
• MGI ID: MGI:7469624
• Synonyms: p21-Cre
• Gene: Igs2 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335288
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Igs2^{tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cdkn1a (MGI:104556)

• Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert a 3,225-bp mouse Cdkn1a (p21) promoter fragment followed by a bicistronic message consisting of the coding sequence for Cre fused to an ERT2 domain, an IRES followed by an ORF coding for enhanced GFP into the intergenic site 2 locus (Hipp11). This construct was microinjected into the pronucleus of recipient zygotes containing attP sites in the Hipp11 locus. Site-specific recombination occurred between the attB sites in the plasmid and attP sites in the H11 genomic locus. Positive embryonic stem cell clones were then implanted into C57BL/6 females. (J:335288)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cdkn1a (mouse) Summary of all recombinase alleles driven by Cdkn1a. Comparative matrix view of recombinase activities Alliance mouse Cdkn1a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 68 strains or lines available

References

• Original: J:335288 Wang B, et al., An inducible p21-Cre mouse model to monitor and manipulate p21-highly-expressing senescent cells in vivo. Nat Aging. 2021 Oct;1(10):962-973
• All: 2 reference(s)