Pold1^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pold1^{tm1b(EUCOMM)Wtsi}
• Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7467227
• Gene: Pold1 Location: Chr7:44182168-44198239 bp, - strand Genetic Position: Chr7, 28.83 cM
• Alliance: Pold1^{tm1b(EUCOMM)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327432
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 44189712 of Chromosome 7 into intron 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted into intron 10 at position 44192163. Exons 4-10 are thus flanked by loxP sites. Cre expression generated a reporter knockout mouse, deleting exons 4-10. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:327432)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pold1 Mutation: 62 strains or lines available

References

• Original: J:327432 Chen T, et al., A hypomorphic mutation in Pold1 disrupts the coordination of embryo size expansion and morphogenesis during gastrulation. Biol Open. 2022 Aug 15;11(8):bio059307
• All: 1 reference(s)