Zranb1^em1Shios
Endonuclease-mediated Allele Detail

Summary

• Symbol: Zranb1^em1Shios
• Name: zinc finger, RAN-binding domain containing 1; endonuclease-mediated mutation 1, Shigeru Oshima
• MGI ID: MGI:7449310
• Synonyms: Zranb1^C443S
• Gene: Zranb1 Location: Chr7:132532905-132588127 bp, + strand Genetic Position: Chr7, 76.32 cM
• Alliance: Zranb1^em1Shios page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: Cysteine codon 443 (TGT) in exon 5 was changed to serine (TCT) (p.C443S) using crRNA (UGAGUCAAGUAAACAAUCUCGUUUUAGAGCUAUGCUGUUUUG), tracrRNA (AAACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCU) and an ssODN template (CGAAGCACCGAGTCTTTGTCATAAATTGCCCCATGTAGCTTGTAGTACTGAGTCAAGTAAAGAATCTCCGGCAGTCCGGTTCCAAAGTGCATATAGTCTACTGTCCAGACGTGTAGCCAACT) with CRISPR/Cas9 technology. The mutation renders the peptide enzymatically inactive. (J:333468)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zranb1 Mutation: 48 strains or lines available

References

• Original: J:333468 Tamura A, et al., Zranb1-mutant mice display abnormal colonic mucus production and exacerbation of DSS-induced colitis. Biochem Biophys Res Commun. 2022 Nov 5;628:147-154
• All: 1 reference(s)