Inka2^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Inka2^{tm1b(KOMP)Wtsi}
• Name: inka box actin regulator 2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7442114
• Gene: Inka2 Location: Chr3:105611915-105628158 bp, + strand Genetic Position: Chr3, 46.44 cM
• Alliance: Inka2^{tm1b(KOMP)Wtsi} page
• IMPC: Inka2 gene page

Mutation origin

• Mutant Cell Line: EPD0805_2_E10
• Germline Transmission: Earliest citation of germline transmission: J:330575
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 105622764 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 105624660. The critical exon(s) is/are thus flanked by loxP sites. Cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:330575)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Inka2 Mutation: 12 strains or lines available

References

• Original: J:330575 Yamada S, et al., Inka2, a novel Pak4 inhibitor, regulates actin dynamics in neuronal development. PLoS Genet. 2022 Oct;18(10):e1010438
• All: 2 reference(s)