Rorc^{em1.1(cre/ERT2)Ayr}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rorc^{em1.1(cre/ERT2)Ayr}
• Name: RAR-related orphan receptor gamma; endonuclease-mediated mutation 1.1, Alexander Y Rudensky
• MGI ID: MGI:7442010
• Synonyms: Rorc^{Venus-T2A-creERT2}
• Gene: Rorc Location: Chr3:94280101-94305583 bp, + strand Genetic Position: Chr3, 40.56 cM, cytoband F2
• Alliance: Rorc^{em1.1(cre/ERT2)Ayr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333738
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Rorc^{em1.1(cre/ERT2)Ayr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rorc (MGI:104856)

• Mutation details: The IRES-Venus-T2A-creER-frt-NeoR-frt cassette was inserted into the Rorc 3-UTR in embryonic stem (ES) cells using the CRISPR/Cas9 system. The resulting chimeras were bred with FLPeR mice to excise the NEO cassette. (J:333738)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rorc (mouse) Summary of all recombinase alleles driven by Rorc. Comparative matrix view of recombinase activities Alliance mouse Rorc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rorc Mutation: 42 strains or lines available

References

• Original: J:333738 Akagbosu B, et al., Novel antigen-presenting cell imparts Treg-dependent tolerance to gut microbiota. Nature. 2022;610(7933):752-760
• All: 1 reference(s)