Mertk^em1Minh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mertk^em1Minh
• Name: MER proto-oncogene tyrosine kinase; endonuclease-mediated mutation 1, Minhong Yan
• MGI ID: MGI:7435744
• Synonyms: MerTK^-
• Gene: Mertk Location: Chr2:128540876-128644814 bp, + strand Genetic Position: Chr2, 62.63 cM
• Alliance: Mertk^em1Minh page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology using sgRNA 5-gGAATGGCCTGTGGTTGACT-3 (5 mismatch indicated by lowercase g) generated mice harboring a sequence with stop codons in all reading frames in exon 2. The stop cassette inserted at genomic position GRCm38/mm10 chr2:128,729,258 has the following sequence: 5-cgcTAAgTGAcTGAccaccgc-3 (Stop codons in upper case, flanking homology sequences are not included). (J:288823)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mertk Mutation: 66 strains or lines available

References

• Original: J:288823 Zhou Y, et al., Blockade of the Phagocytic Receptor MerTK on Tumor-Associated Macrophages Enhances P2X7R-Dependent STING Activation by Tumor-Derived cGAMP. Immunity. 2020 Feb 18;52(2):357-373.e9
• All: 2 reference(s)