Adgrf5^tm1.2Soff
Targeted Allele Detail

Summary

• Symbol: Adgrf5^tm1.2Soff
• Name: adhesion G protein-coupled receptor F5; targeted mutation 1.2, Stefan Offermanns
• MGI ID: MGI:7431399
• Synonyms: Gpr116^null
• Gene: Adgrf5 Location: Chr17:43671342-43770448 bp, + strand Genetic Position: Chr17, 19.73 cM
• Alliance: Adgrf5^tm1.2Soff page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245698
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites flank exons 8 of the Adgrf5 gene. An frt-flanked neomycin selection cassette was removed via FLP1-mediated recombination in the germline. he F1 generation mice were crossed with EIIa-Cre mice to generate a null allele by recombining loxP sites in the early embryonic stage. (J:245698)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adgrf5 Mutation: 73 strains or lines available

References

• Original: J:245698 Lu S, et al., Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4). PLoS One. 2017;12(8):e0183166
• All: 1 reference(s)